Differential Drug Sensitivity Analysis for Two-Condition High-Throughput Screens

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Documentation for package ‘diffHTS’ version 0.1.0

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annotate_hit_info Annotate hits with compound metadata
apply_plate_layout Stamp a plate-map layout onto measured readings
baseline_subtract Subtract the blank background signal from a plate
build_auc_matrix Build a compound-by-sample AUC matrix
calculate_qc_metrics Plate-level quality-control metrics for high-throughput screens
calc_cv Control coefficient of variation per plate
calc_drc_auc Area under the dose-response curve
calc_plate_qc Comprehensive per-plate quality assessment
calc_replicate_correlation Pairwise replicate correlation between plates
calc_replicate_cv Replicate coefficient of variation per compound
calc_robust_z_prime Robust Z'-factor per plate
calc_sb_ratio Signal-to-background ratio per plate
calc_sn_ratio Signal-to-noise ratio per plate
calc_ssmd SSMD per plate (strictly standardised mean difference)
calc_well_zscore Per-well standard and robust Z-scores
calc_z_factor Z-factor per plate (sample-aware)
calc_z_prime Compute per-plate Z' factors
check_control_label Validate control-well labelling
clean_compound_id Standardise compound identifiers
clean_filename Sanitise a string for use as a file name
cluster_auc_matrix Hierarchically cluster an AUC matrix
coef.dsr_4pl Fit a four-parameter logistic dose-response model
compute_auc Area under a four-parameter logistic dose-response curve
compute_delta_auc Differential (delta) AUC between two screening conditions
convert_conc_log10 Convert concentrations to a log10 scale
detect_outlier_wells Flag outlier wells by the IQR rule
export_hit_table Export a hit summary table
extract_cluster_hit Extract the most active compound cluster
extract_drc_params Extract dose-response parameters
filter_bad_replicate Drop compounds with poor replicate reproducibility
filter_low_quality_curve Drop low-quality dose-response curves
filter_valid_plates Keep only plates that pass the Z' threshold
fitted.dsr_4pl Fit a four-parameter logistic dose-response model
fit_4pl_curve Fit four-parameter logistic curves per compound
fit_dose_response Fit a four-parameter logistic dose-response model
flag_qc_plates Flag plates that fail quality-control thresholds
four_pl Four-parameter logistic (4PL) dose-response function
generate_hts_report Generate an HTML screening report
hts_compound_meta Example compound metadata
hts_dose_response Example gradient dose-response secondary screen
hts_pal Colourblind-safe qualitative palette for diffHTS
hts_plate_384 Example single 384-well screening plate (realistic layout)
hts_primary_raw Example single-concentration primary screen (raw signals)
import_dose_response Import gradient dose-response data
merge_plate_data Combine normalised plates into one data set
norm_by_control Normalise wells to plate controls
plate_layout Standard microplate coordinate map
plate_layout_1536 Standard microplate coordinate map
plate_layout_384 Standard microplate coordinate map
plot_auc_heatmap AUC clustering heatmap
plot_batch_drc_overlay Overlay multiple dose-response curves
plot_cluster_tree Plot the compound clustering dendrogram
plot_condition_scatter Scatter plot comparing two conditions or cell lines
plot_control_scatter Scatter plot of control readouts across plates
plot_cv_distribution Histogram of replicate CV values
plot_delta_auc_heatmap Heatmap of differential AUC across conditions or cell lines
plot_dose_response_curves Plot dose-response curves for two conditions
plot_hit_bar_count Hit-count bar chart
plot_hit_stratify_bar Stratified hit-count bar chart
plot_ic50_auc_cor IC50 versus AUC scatter
plot_inhibition_hist Histogram of compound inhibition on a plate
plot_plate_heatmap Plate-layout heatmap of well readouts
plot_plate_heatmap_inhibition Percent-inhibition plate heatmap
plot_plate_heatmap_raw Raw-signal plate heatmap
plot_plate_qc Visualise any plate quality-control metric
plot_primary_inhibition_rank Ranked primary-activity curve
plot_primary_secondary_cor Primary versus secondary activity correlation
plot_qc_boxplot Quality-control boxplot of control wells across plates
plot_qc_circular_heatmap Circular heatmap of control readouts across plates
plot_qc_metric_circular Circular plot of a per-plate QC metric
plot_qc_metric_trend Trend plot of a QC metric across plates
plot_replicate_scatter Replicate scatter plot(s) with regression fit and correlation
plot_sigma_hits Distribution plot of sigma-based hits
plot_single_drc Plot a single dose-response curve
predict.dsr_4pl Fit a four-parameter logistic dose-response model
print.dsr_4pl Fit a four-parameter logistic dose-response model
rank_hit_compound Rank and stratify hit compounds
read_hts_plate Import a plate readout file into a standard HTS object
read_plate_layout Read a user-defined plate-map layout file
residuals.dsr_4pl Fit a four-parameter logistic dose-response model
screen_delta_auc Example differential-AUC hit table
screen_doseresponse Example two-condition dose-response screen
screen_plate_layout Example 96-well plate layout with well positions
screen_plate_qc Example per-plate quality-control summary
select_hits_cutoff Select hits by an absolute cut-off across conditions
select_hits_sigma Select hits by a sigma (z-score) threshold
select_primary_hit Select primary-screen hits
select_sigma_hits Select primary-screen hits by distance from the library distribution
summarize_plate_setup Summarise how each plate in a run is set up
summarize_primary_hit Summarise primary-screen hits
summarize_sigma_hits Summarise sigma-based hits
theme_hts Modern academic ggplot2 theme for diffHTS figures
windows_to_linux_path Convert a Windows path to a forward-slash path